Enzyme lab

Determining Properties of an Enzyme Purpose: Enzymes serve as proteins that influence the billhook of a answer. Enzymes are long chains of amino acids with net shapes. There a variety of enzymes, all(prenominal) with their own righteousness and job. The dynamical site on an enzyme is specific to a single substrate, which tail end activate the energy in the enzyme. Without enzymes normal metabolic reactions would be sluggish. The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The tack of temperature and pH depart be testinged and their influence on the enzyme recorded. If the environment is cold, then enzymes volition not activate and if pH is game, then enzymes will not activate. This I believe because at low temperatures reactions take to the woods to slow down and at high pH allthing will denature. Techniques: Enzyme activity was determined in sevensome test tubes apiece with a distinguishabl e combination of buffers, H202, extract, and dye. sort tubes were primed(p) in a mass spectrometer and their absorbance recorded. Absorbance was recorded at twenty dollar bill second intervals for two full minutes. A temperature effect on enzymes was heedful by exposing test tubes to four different temperature environments. (4C, elbow room temperature, 32C, and 48C).Seven test tubes were assembled exactly the same as the sample before.

once more the test tubes were placed in a spectrometer and their absorbance was measured at intervals of twenty seconds for two minutes. The effect of pH on an enzyme was tested in nine test tubes, each with a different c! ombination of, buffer pH, H202, extract, and dye. Again the test tubes were placed in a spectrometer and their absorbance recorded every twenty seconds for a full two minutes. Results: Enzyme Activity Time (sec) underground 2 & 3 .5mL ExtractTube 4 & 5 1.0mL ExtractTube 6 & 7 2.0mL Extract 20.11.011.4 40.11.011.9 60.11.012 80.12.012 100.12.012 120.12.012 Temperature effect on Enzyme Time (sec)Tube 3 & 4 4C...If you want to number a full essay, order it on our website: OrderCustomPaper.com

If you want to get a full essay, visit our page: write my paper